Crystal violet in the histochemical diagnosis of Helicobacter pylori
DOI:
https://doi.org/10.46502/issn.2710-995X/2023.9.02Palavras-chave:
Helicobacter Pylori, histochemistry, Crystal violet stain, Giemsa stain.Resumo
Helicobacter pylori infection is the main causative agent of gastric cancer worldwide. With the objective of evaluating the effectiveness of the use of the histochemical technique of Crystal violet in the determination of H. pylori in stomach biopsies, a retrospective study of technological development was carried out in the Pathological Anatomy departments of the General Hospital "Dr. Juan Bruno Zayas Alfonso" and TOXIMED, using samples obtained in the period from January to December 2018 from patients with a gastric biopsy with positive H. pylori by the Giemsa method, treated at the Gastroenterology Clinic of the General Hospital "Dr. Juan Bruno Zayas Alfonso”. In the investigation, the value of Sensitivity, Specificity, Positive and Negative Predictive Value of the Crystal violet technique and the Accuracy corresponded to 1. The results obtained with the Crystal violet technique, in comparison with the Giemsa technique, have shown that this diagnostic test It has similar accuracy and feasibility parameters, so it can be used as an alternative diagnostic method.
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